Review



microarray experiment design, setup, and data  (Biotechnology Information)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Biotechnology Information microarray experiment design, setup, and data
    Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
    Microarray Experiment Design, Setup, And Data, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray experiment design, setup, and data/product/Biotechnology Information
    Average 90 stars, based on 1 article reviews
    microarray experiment design, setup, and data - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "Integrated microarray and multiplex cytokine analyses of Kaposi's Sarcoma Associated Herpesvirus viral FLICE Inhibitory Protein K13 affected genes and cytokines in human blood vascular endothelial cells"

    Article Title: Integrated microarray and multiplex cytokine analyses of Kaposi's Sarcoma Associated Herpesvirus viral FLICE Inhibitory Protein K13 affected genes and cytokines in human blood vascular endothelial cells

    Journal: BMC Medical Genomics

    doi: 10.1186/1755-8794-2-50

    Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and microarray data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
    Figure Legend Snippet: Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and microarray data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.

    Techniques Used: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Western Blot, Plasmid Preparation, Expressing, Reverse Transcription Polymerase Chain Reaction, Microarray



    Similar Products

    microarray experiment design, setup, and data  (Biotechnology Information)
    90
    Biotechnology Information microarray experiment design, setup, and data
    Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and <t>microarray</t> data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.
    Microarray Experiment Design, Setup, And Data, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microarray experiment design, setup, and data/product/Biotechnology Information
    Average 90 stars, based on 1 article reviews
    microarray experiment design, setup, and data - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and microarray data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.

    Journal: BMC Medical Genomics

    Article Title: Integrated microarray and multiplex cytokine analyses of Kaposi's Sarcoma Associated Herpesvirus viral FLICE Inhibitory Protein K13 affected genes and cytokines in human blood vascular endothelial cells

    doi: 10.1186/1755-8794-2-50

    Figure Lengend Snippet: Validation of gene expression array data by qRT-PCR and western blotting . (a) . Eleven genes were randomly picked and their relative mRNA levels in the mock- and 4OHT- treated vector and K13-ER TAM expressing HUVECs were examined using real-time RT-PCR. The two different methods, the microarrays and the RT-PCR, show excellent qualitative agreement. (b) . Linear regression analysis shows excellent correlation between the qRT-PCR and microarray data with a coefficient of variation (R 2 ) of 0.845. (c) . Western blot analysis confirms induction of COX-2/PTGS2 upon treatment of K13-ER TAM cells with 4OHT. Tubulin blot shows equal protein loading.

    Article Snippet: The microarray experiment design, setup, and data have been deposited in National Center for Biotechnology Information's Gene Expression Omnibus and are accessible through GEO series accession number GSE16051.

    Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Western Blot, Plasmid Preparation, Expressing, Reverse Transcription Polymerase Chain Reaction, Microarray